No, you only need to apply once.
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AWS Promotional Credits for hosting most Alexa Skills for free
I’m not eligible yet. Do I need to wait to apply?
How do I know if I am eligible to receive AWS promotional credits?
How are AWS promotional credits expended on my account if I have more than one credit?
Where can I find my Vendor ID and my AWS Account ID?
I made a mistake filling out the application, what should I do?
Can credits be split across multiple AWS accounts?
What services can I use my AWS promotional credit on?
I don’t incur charges related to Alexa skills. Can I still use my credit for other AWS usage charges or give it to a friend?
What counts as usage for determining my eligibility for the monthly AWS Promotional Credit?