No, you only need to apply once.
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AWS Promotional Credits for hosting most Alexa Skills for free
I am eligible for AWS promotional credit but did not receive it. What happened?
I submitted the application but didn’t get an email confirming my submission within the next couple days. Why is this?
What happens if a skill gets suppressed because of policy violation, developer request, etc.?
What happens if I change my contact information after submitting the AWS Promotional Credits application?
I updated my developer account's company email address, but the promotional code was still sent to the old email address. What do I do?
What qualifies as having a live skill?
I’m not eligible yet. Do I need to wait to apply?
How do I know if I am eligible to receive AWS promotional credits?
How are AWS promotional credits expended on my account if I have more than one credit?